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产品目录

HpaII内切酶图片
产品货号:
GS2241
中文名称:
HpaII内切酶
英文名称:
HpaII
产品规格:
1kU
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)

技术规格

Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sitesC↑CGG
来源E.coli strain,that carries the cloned gene Hpa II from Haemophilus parainfluenzae
Assayed onLambda DNA
Optimal buffer10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;1 mM DTT.
Optimal temperature37℃
Storage conditions10mMTris-HCl(pH 7.5);50 mM NaCl;0,1 mM EDTA;1 mM DTT;100 μg/ml BSA;50%glycerol;Store at -20℃.
LigationsAfter 10-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1μgof Lambda DNA with 10 u.a.of enzyme for 16hoursat 37℃.
Methlation effectBlocked by CG methylation.
Thermal inactivationenzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible EndsBsiSI,HpaII,MspI

相关搜索:HpaII内切酶HpaII限制性内切酶
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